
Ryan Naicker
Laboratory Technician

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About me
Master of Science in Microbiology
Education

University of KwaZulu-Natal
2021 - 2024Master of Science Microbiology 75%Title: Investigating the quorum sensing and biofilm inhibitory potential of sponge-associated bacteria-derived crude biosurfactant extracts and their biosynthesised nanoparticlesKey objectives include: The evaluation of sponge-associated bacteria for their biosurfactant synthesizing potential using several screening techniques. The biosynthesis of metallic and biopolymer nanoparticles using biosurfactants, followed by their physio-chemical characterization to determine their… Show more Title: Investigating the quorum sensing and biofilm inhibitory potential of sponge-associated bacteria-derived crude biosurfactant extracts and their biosynthesised nanoparticlesKey objectives include: The evaluation of sponge-associated bacteria for their biosurfactant synthesizing potential using several screening techniques. The biosynthesis of metallic and biopolymer nanoparticles using biosurfactants, followed by their physio-chemical characterization to determine their synthesis, molecular and elemental composition, zeta potential, surface charge, particlesize and distribution, and topographical features. The evaluation of biosurfactants and their respective nanoparticles for their bioactive potential using antimicrobial susceptibility testing, quorum sensinginhibition assays targeting intraspecies and interspecies communication, andinitial/mature biofilm inhibition assays. Show less

University of KwaZulu-Natal
2013 - 2017Bachelors of Science Microbiology and Genetics 70% AverageDeans Commendation (2014 and 2016) Certificate of Merit in MICR 306: Advanced Applications of Fungi and Viruses (2016)

University of KwaZulu-Natal
2017 - 2017Bachelors of Science Honours Microbiology 74%Activities and Societies: Research project presented at the South African Society for Microbiology (SASM) conference - Regional Research Project - Quorum Sensing Inhibitory Effects of Copper Nanoparticles Biosynthesized using Sponge-Associated BacteriaModules:Specialized Microbial Molecular Techniques Bacteriology (Certificate of Merit)MycologyMolecular Genetics Biotechnology
Experience

Clinical Research Labs
Apr 2018 - May 2019Laboratory Technician Project manager: Led the WHICH Injection Study, collaborating with three clinical sites in Cape Town, East London, and Durban. Responsibilities included project planning, budgeting projections, supplies management, training, and addressing queries. Sample processing: Processed peripheral blood mononuclear cells (PBMCs), cytobrushes, blood, and urine and conducted rapid tests. Data management: Captured data on Laboratory Data Management Systems (LDMS) and Laboratory Information Management Systems (LIMS) databases. Ensured quality assurance of all samples recorded in the databases and stored in the biorepository Captured data on Laboratory Data Management Systems (LDMS) and Laboratory Information Management Systems (LIMS) databases. Logistics coordinator: Coordinated and approved shipments of samples from the biorepository to local and international destinations. Courier management and training: Oversaw and trained couriers, monitoring trip times, mileage, and competency. Wrote standard operating procedures (SOPs) to ensure standardized protocols and processes. Managed and monitored laboratory equipment including -80°C ultra-freezers, liquid nitrogen storage tanks, -20 °C freezers, and lab temperatures using Omniflex. Generated invoices for completed work and services provided to clients. Show less

Global Water Labs
Sept 2019 - Jun 2020Microbiology Analyst Ensuring compliance with the organizations Quality Management System and ISO 17025:2017 standard. Conducted water sample testing using the Membrane Filtration Methods (Escherichia coli, Total coliforms, Faecal coliforms and Legionella species) and the Heterotopic Plate Count Method. Analyzed and interpreted results to ensure water quality standards. Wrote and reviewed procedures, work instructions, and forms, ensuring accuracy and adherence to standards. Conducted root cause analysis for non-conformances, implementing corrective actions to improve processes and prevent recurrence. Participated in and successfully passed proficiency testing schemes such as the National Laboratory Association (NLA) and IFM Quality Services. Ensured the accuracy and reliability of laboratory tests by preparing and verifying media and reagents, as well as verifying the accuracy of micropipettes, dispensers, timers, and thermometers. Maintained an equipment schedule for laboratory equipment service and calibration dates, coordinating with service providers and calibration services as needed. Liaised with service providers and calibration services for quotations and equipment concerns, ensuring timely maintenance and repair of laboratory equipment. Maintaining an equipment schedule with regards to the service and calibration dates of all laboratory equipment. Liaising with service providers and calibration services for quotations and equipment concerns . Assisted in document control and filing of laboratory equipment records, maintaining organized and accessible documentation for regulatory compliance. Show less

University of KwaZulu-Natal (School of Life Sciences)
Aug 2022 - May 2024Semester Based Module: Introduction to Fungi and Viruses Conducted tutorials and pre-practical talks, provided instruction, and facilitated discussions to enhance student understanding. Assessed and marked student tests and tutorials, ensuring accuracy and fairness in grading. Provided administrative support in collating, checking, and maintaining student marks. Moderated assessments conducted by demonstrators to uphold assessment standards. Maintained demonstrator preparedness before practical sessions, ensuring smooth operations and adherence to protocols. Show less
Laboratory Demonstrator
Mar 2021 - May 2024Teaching Assistant
Aug 2022 - Dec 2022

University of KwaZulu-Natal (School of Life Sciences)
Oct 2024 - Dec 2024Research Assistant• Planned, prepared and executed assays to meet research project deadlines.• Conducted enzyme mimetic activity assays for over 150 nanoparticles, focusing on haloperoxidase, superoxide dismutase (SOD), catalase, and oxidase activities: Haloperoxidase activity was assessed using Phenol-red Bromination and Celestine Blue assays and associated bactericidal activity was evaluated using colony count assays with marine and clinical indicator organisms. Evaluated quorum quenching potential of nanoparticles using AHL degradation assays with Chromobacterium violaceum biosensor organisms. Catalase and oxidase activities were evaluated using the Amplex Red reaction kit, while SOD activity was measured with a SOD determination kit.• Investigated quorum sensing inhibition potential of extracts and nanoparticles through quantitative AI-2 bioluminescence assays with two Vibrio species biosensors.• Conducted optimization trials for a biofilm haloperoxidase assay using clinical and marine indicator organisms.• Prepared glycerol stocks of over 100 pure Tenacibaculum sp. cultures, biobanked at -80 °C.• Performed gel electrophoresis and gel documentation imaging of genes amplified by PCR.• Prepared bacterial extracts for downstream chemical analysis using fermentation and solvent extraction techniques.• Recorded detailed observations and experimental results for accurate reporting. Analyzed experimental data, interpreted findings, and resolved technical issues to optimize procedures.• Mentorship: Provided technical guidance and support to postgraduate students.• Maintained cleanliness and organization of the laboratory. Show less
Licenses & Certifications

Conference Presenter
South African Society of Microbiology (National)Sept 2023
Use of Software SPSS Workshop
University Capacity Development Programme (UCDP)Aug 2023
CAES Analysis and Interpretation of Data Workshop
University Capacity Development Programme (UCDP)Jul 2023
Fire Fighting Level I
KDS Health and Safety ConsultantsNov 2018
Good Clinical Practice (GCP) Course
Clinical Trials Consultants - Dr. S. N. GovenderApr 2018.webp)
Mycobacterial Genetics Course
SANTHE (Sub-Saharan African Network for TB/HIV Research Excellence)Jul 2016
Real-Time PCR Applications Training Course
Thermo Fisher ScientificJun 2023
Demonstrator-Teaching Assistant Training Workshop
University of KwaZulu-NatalFeb 2022
IATA Class 6.2 and Class 9 Course
BiocairJul 2018
ISO 22000 (HACCP and PRPS) for Food Safety
UdemyMay 2024
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