Matthew D.

Experience

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  Celgene

  May 2016 - Aug 2016

  Celgene, Biologics Technical Operations Intern

  • Collaborated with Biologics API Drug Substance Group on process engineering for Stage II Drug Development• Developed process flow spreadsheet mapping upstream and downstream unit operations• Performed statistical analysis on process parameters using Excel and R

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  Northeastern University

  Sept 2016 - Apr 2017

  Bioengineering Tutor
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  Visterra Inc.

  Jul 2017 - Dec 2017

  Visterra Inc, Therapeutic Antibody Research Co-op

  • Validated the biochemical and biophysical attributes of therapeutic mAbs for the treatment of IgA Nephropathy• Expressed recombinant antibodies, purified via FPLC, and characterized using ELISA-based assay formats• Optimized quantitation, binding kinetics, and epitope binning of proteins using biolayer interferometry (on Octet)• Assessed mechanism of action of recombinant antibodies binding to multiple myeloma cells using flow cytometry

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  Northeastern University

  Jan 2018 - Jun 2018

  Slavov Lab, Undergraduate Student Researcher

  • Determined if cell-specific ribosomal protein profiles exist in monocytes, macrophages, and dendritic cells• Used sucrose gradient separation for polysome profiling; analyzed isolated fractions by mass spectrometry• Optimized process of differentiating monocytes into macrophages and dendritic cells• Validated phenotypes & biomarker distributions of these cells via phase contrast microscopy & flow cytometry

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  Harvard Medical School

  Jul 2018 - Feb 2019

  McCarroll Lab, Research Assistant Intern

  • Extensive experience in single-cell RNA sequencing using Drop-Seq and 10x Genomics platforms• Independently researched and developed a protocol to fix and permeabilize single-cell suspensions, allowing long-term sample storage while preserving RNA integrity and intra- & extra-cellular antigen sites.• Validated this protocol using electrophoresis, immunofluorescence, and 10x Genomics single-cell RNA-seq kit• Worked on developing method to fix/permeabilize cells, label intra- & extra-cellular antigens with DNA-conjugated antibodies, and simultaneously quantify proteins and RNA by single-cell RNA-seq readouts Show less

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  Embr Labs

  Jul 2019 - Dec 2019

  Embr Labs, Biomedical Engineer Intern

  • Developed a method to improve the heating and cooling ability of a personal thermoelectric device embedded in a wristband, informing key product design choices• Performed these experiments by writing a LabVIEW program to record and plot heat flux data from the device, and integrated this with a Python script to control the device.