Dianna Y. Olukotun M.S, ASP, RBP

Experience

• 

  The University of Texas at San Antonio

  Dec 2006 - Aug 2008

  Tech Staff II

  • Collected water and sediment samples and filtrated and plated samples utilizing aseptic techniques. • Studied water samples to screen for environmental markers of contamination.• Performed water filtration plating on different types of agar such as: R2A, KF, and MacConkey.• Plate culturing of microbial organism: E.coli, streptococci, etc. • Collected water samples from wells throughout San Antonio for SAWS screening for bacterial hits or point sources of contamination in the water system. • Plated and grew organisms for later extraction and to find bacterial genetic markers.• Researched studies utilizing journals and books, documented research findings.• Worked with PCR and Electrophoresis• Knowledge in slide preparation Show less

• 

  UT Health San Antonio

  Jun 2009 - Sept 2013

  • Western Blotting of cells• Culturing and drug treating cell lines• Blood work: Separation and Purification• Keeping lab organized• Lab supplies and equipment ordering• Adhering to laboratory compliance rules• Keeping machinery in good working order• Measuring samples with Nanodrop• Measuring samples with BioAnalyzer• Basic knowledge of Flow cytometry (FACS)• Using ViaCell for cell viability assays• Running IC50 studies on cancer drugs and MTTs.• RNA and DNA Isolation from cells • Preparing and making cDNA of samples. • Working with mice in a laboratory setting• RT-PCR• Data presentation from experiments Show less • Collected brain specimens for brain bank• Aiding in the design and execution of experiments• Blood Processing• DNA Isolation from tissue for genotyping• RNA Isolation from brain tissue• Making cDNA • Measuring RNA samples with Bioanalyzer• Measuring samples with Nanodrop• Immunoprecipitation and Western Blotting of brain tissue• Use of cryostat machine• Measuring pH from brain tissue• Keeping brain database updated• Keeping lab organized• Data presentation from experiments• Lab supplies and equipment ordering• Dot Blotting• RT-PCR • Experience supervising laboratory volunteers and graduate students. • Cell culturing Show less

  • Senior Research Technician/ Lab Manager

    Oct 2011 - Sept 2013

  • Senior Research Assistant

    Jun 2009 - Sept 2011
• 

  The University of Texas at San Antonio

  Sept 2013 - Oct 2022

  • Evaluating BSL-1 and BSL-2 labs • Data entry • Adhering to laboratory compliance rules• Collaborating with laboratories• Following Chemical Inventories in school laboratories for compliance• Helping measure and follow radiation levels in labs• Part of the HazMat team• Molecular Biology article research and methodology

  • Biosafety Officer

    Mar 2022 - Oct 2022

  • Radiation and Laser Safety Coordinator

    Feb 2018 - Apr 2022

  • EHSRM/Safety Specialist II

    Sept 2013 - Jan 2018
• 

  Environmental Health & Engineering

  Nov 2022 - Oct 2023

  Biosafety Officer
• 

  Astellas Pharma US

  Oct 2023 - now

  EH&S Manager