Rohith Karunakaran

Experience

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  Akums Drugs& Pharmaceuticals Ltd

  Jun 2022 - Aug 2022

  Production Process Engineer

  • Observed and participated in the manufacturing and export processes of pharmaceutical formulations, covering various dosage forms (oral liquids, sterile injectables, food supplements, nutraceuticals, and animal health care products).• Acquired skills in quality control, adhering to Good Manufacturing Practices (GMP), and robust understanding of pharmaceutical production techniques.• Experienced in working at a biopharmaceutical company, consistently adhering to professional ethics, and diligently following standard protocols. Show less

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  Indian Institute of Technology, Madras

  Sept 2022 - Apr 2023

  Project Intern

  • Conducted research on bacterial strains in agar culture medium, analyzing their chemotaxis growth patterns.• Investigated the impact of Tetracycline and Norfloxacin antibiotics on bacterial growth and assessed chemotaxis movement through antimicrobial susceptibility tests.• Performed advanced microfluidic systems, including Lab-on-Chip technology, for real-time analysis of bacterial chemotaxis in the presence of antibiotics, with a focus on genotypic analysis of antibiotic resistance.• Acquired experience to culture all types of bacterial strains and interpreted chemotaxis movement. Show less

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  Winpath Lab & Health Care

  May 2023 - Jul 2023

  Internship Trainee

  • Engaged in clinical assessments and diagnostic tests within a fast-paced laboratory environment, gaining hands-on experience in sample collection, processing, and analysis.• Demonstrated attention to detail and adherence to quality control protocols, contributing to the delivery of diagnostic results.• Performed various clinical diagnostic tests, examined various samples and interpreted results.

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  Northeastern University, Bouvé College of Health Sciences

  Nov 2023 - Aug 2024

  Graduate Research Assistant

  Dr. Loring’s Lab• Optimized DNA-to-XtremeGene ratios for BOSCH 23 cell transfections using alpha-7 and 5HT3 chimera constructs, achieving high transfection efficiency and low cytotoxicity.• Investigated Ric3 and TMEM35 (NACHO) roles in alpha-7 receptor expression and functionality using advanced Western blotting techniques to assess protein expression levels.• Increased iodination efficiency by 15% using Gel Exclusion Chromatography, improving the quality and reliability of I-125 labeled Alpha-Bungarotoxin binding assays.• Analyzed IL6 and TNF-alpha induced signaling pathways by monitoring alkaline phosphatase secretion as a pathway activation marker in HEK-Blue cells.• Conducted Ex-vivo studies by mapping the alpha-7 receptor expression in mouse brain sections using I-125 labeled Alpha-Bungarotoxin; compared receptor distribution and binding affinity in wild-type, Ric3-knockout, and TMEM35-knockout models. Show less

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  Northeastern University

  Jan 2024 - Apr 2024

  Graduate Research Assistant

  The Joshi Lab - Engineered Living Materials• Managed E. coli cultures in a stirred-tank bioreactor to enhance growth conditions and maximize biomass production.• Employed advanced filtration methods to isolate the curli fibers from other cell organelles, ensuring high purity and quality.• Utilized the curli fibers in manufacturing of Engineered Living Materials using BIND Technology.

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  Center for Drug Discovery at Northeastern University

  Feb 2024 - Dec 2024

  Graduate Research Assistant
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  Solid Biosciences

  Jan 2025 - now

  Associate Scientist, Immunoassay Development Co-Op