Alessandro Rapone

Education

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  Alma Mater Studiorum - Università di Bologna

  2020 - 2022

  Master's Degree Molecular and Cell Biology 110/110 with honors

  Nine months internship in molecular and cell Neurobiology in professor Barbara Monti's Lab. Thesis title "AGC1 Deficiency: Crosstalk between metabolism and epigenetic modification" my tasks were - Cell culture (both immortalized and stem cells)- Characterization of sirtuin expression in AGC1 Deficiency cell models.- Study of the effect of BCAA, ketone bodies and nicotinamide riboside supplementation on proliferation and H3 acetylation status in AGC1 Deficiency cell… Show more Nine months internship in molecular and cell Neurobiology in professor Barbara Monti's Lab. Thesis title "AGC1 Deficiency: Crosstalk between metabolism and epigenetic modification" my tasks were - Cell culture (both immortalized and stem cells)- Characterization of sirtuin expression in AGC1 Deficiency cell models.- Study of the effect of BCAA, ketone bodies and nicotinamide riboside supplementation on proliferation and H3 acetylation status in AGC1 Deficiency cell models.Techniques learnt:- SDS-PAGE Western Blot- Cell cultures (both immortalized and stem cells)- Immunofluorescence - Supplementations and proliferation study- MTT essay - DNA extraction - PCR- Real Time PCR- Nitric oxide assay Show less

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  Università di Roma Tor Vergata

  2017 - 2020

  Bachelor's Degree Biology 110/110 With Honors

  Three months internship in Cellular Biology in Stefano Cannata's lab. Thesis title: Neuromuscolar junction in microfluidic chip: study on assonal elongation