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OUAD SOLTANI

OUAD SOLTANI

Trainee

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  • Timeline

  • About me

    PhD student

  • Education

    • Institut national d'Agronomie

      2009 - 2014
      Engineer's degree Plant Molecular Biology with honors

    • Ruhr University Bochum

      2022 - 2024
      Postdoctoral researcher Plant Molecular Biology

      Activities and Societies: laboratory work, tracking scientific records, writing reports, giving talks, teaching, supervision of bachelor and master students My work focuses primarily on nutrient metals (such as iron, zinc, copper) and toxic heavy metals (cadmium) and on the functional characterization of metal related genes (NRAMPs) using a combination of molecular biology approaches such as cloning, functional complementation of wild type and yeast mutants, Electrophoretic Mobility Shift Assay (EMSA) for protein-DNA interaction studies, GFP-tagged fusion proteins and confocal microscopy for subcellular localisation of proteins.

    • University of Strasbourg

      2015 - 2017
      Master's degree Molecular Biology with honors

      Activities and Societies: internship at INRA Versailles

    • University of Potsdam

      2017 - 2022
      Doctor of Philosophy - PhD Plant Genetics

      Activities and Societies: qPCR, digital PCR, yeast two-hybrid screening, BiFC, FRET, Co-IP, laser scanning microscopy, R programming, Next-Generation Sequencing (NGS), image analysis, CRISPR-Cas9 gene editing, managing growth chambers, green house and field experimental conditions. Developed high-throughput screening tests to study genetic control of plant growth, with applications in agriculture. Utilized advanced biophysical and biochemical techniques, including CRISPR, BiFC, FRET, and Co-IP, in-vivo protein ubiquitylation assay to support structural biology research in crop development. Performed biochemical and cellular tests with different detection methods, including fluorescence and luminescence. Managed projects and worked with international research… Show more Developed high-throughput screening tests to study genetic control of plant growth, with applications in agriculture. Utilized advanced biophysical and biochemical techniques, including CRISPR, BiFC, FRET, and Co-IP, in-vivo protein ubiquitylation assay to support structural biology research in crop development. Performed biochemical and cellular tests with different detection methods, including fluorescence and luminescence. Managed projects and worked with international research teams.Published research findings and presented them at conferences. Show less

  • Experience

    • National Agronomic Institute

      Jan 2014 - Feb 2017
      Trainee

      6 month internship in the group of Pr Sonia Hamza about the Validation of two SSR markers for the résistance to Septoria tritici in a Tunisian durum wheat variety 'Agili39'

    • INRA French National Institute for Agricultural Research

      Feb 2015 - Feb 2017

      6 month internship in the group of Pr. Thomas Girin at the group of Nitrate signalling, transport and utilization. My project was about the role of BdNLP6 and BdNLP7 transcription factors in the regulation of Primary Nitrate Response in Brachypodium distachyon internship in the group Membrane dynamics and plant Microorganism Interactions under the supervision of Pr. Karim Bouhidel, about the study of plasma membrane desensitization of tobacco cells in response to defense reaction elicitors

      • Trainee

        Feb 2016 - Feb 2017

      • Trainee

        Feb 2015 - Feb 2017

    • University of Potsdam

      Feb 2017 - now
      Researcher PHD Student

      studying the genetic control of leaf growth in Barley

    • Ruhr University Bochum

      Oct 2022 - now
      Postdoctoral Researcher

  • Licenses & Certifications

    • Clinical Research Associate Certification (CRA)

      VIARES
      May 2022