Douglas Tremblay

Experience

• 

  EcoScience Laboratories

  Jan 1986 - Jan 1990

  Assistant Scientist I

  • Isolated, cultured, and characterized soil bacteria and fungi. Tested isolates for efficacy against insects.• Developed promising candidates for use as biological control agents.

• 

  Maine Department of Marine Resources - Dept. of Shellfish Sanitation

  Jan 1990 - Jan 1993

  Microbiologist I

  • Managed the microbiology laboratory for the Shellfish Sanitation Program and supervised two full-time employees. • Maintained the laboratory in good standing with all FDA regulations and safety requirements.

• 

  University of Tennessee

  Jan 1992 - Jan 1996

  Graduate Teaching Assistant

  Financed graduate school by teaching 2-3 sections of General Biology Laboratory to undergraduate students each semester.

• 

  North Carolina State University

  Jan 1996 - Jan 2001

  Research Associate III - Department of Plant Pathology

  • Researched and characterized the Peppermint stunt vitivirus. -Developed an RNA-based detection method for Peppermint stunt virus that enabled detection and quantitation of the virus in peppermint plants.• Completed a research project that characterized mutated Red clover necrotic mosaic virus movement proteins and revealed their localization pattern.• Ensured that the laboratory was in compliance with radiological, biological, and chemical safety requirements.

• 

  BASF Global BioSciences

  Jul 2001 - Sept 2016

  PROTEIN ANALYTICS & REGULATORY (2012-2016)• Used biotechnology tools such as the Minifors fermenter to express recombinant proteins, including highly hydrophobic membrane proteins in E. coli.• Purified recombinant proteins using AKTA FPLC, Bio-Rad Prep Cell, immunopurification, and other methodologies and techniques.• Characterized proteins using a range of techniques including protein and enzyme assays and digestibility studies.• Developed protocols and SOPs and GLP validated procedures for the quantitative analysis of proteins in plant tissues using the Protein Simple capillary electrophoresis system.• Introduced and implemented new assays for the quantification and characterization of proteins that have facilitated deregulation for two agronomics related products (one of them is not yet complete). • Designed LIMS database workflows to store data derived from the characterization of recombinant proteins. Collaborated with developers to implement, troubleshoot and improve the database.• Workshops include:- The Seven Habits of Highly Effective People: Franklin Covey Co. Show less SCIENTIST ─ CROP PHYSIOLOGY & PHENOTYPING (2001-2012)• Designed, implemented and documented phenotypic screens for the study of crops and model plants in a team-oriented environment. Served as the chief technician for the LemnaTec plant phenotyping system. - Screens included Genome-Wide Association Mapping of maize inbreds and analysis of transgenic crops and model species. -Traits investigated included resistance to drought, cold, and oxidative stress and above and below ground biomass. • Performed statistical analysis of research data and communicated project updates in internal review meetings.• Established a new high-throughput plant phenotyping system for crops and model plants that greatly increased the number of phenotypes that were measured while decreasing labor requirements by over 50%.• Designed and validated a screen to test the resistance of recombinant soybean plants to fungal root pathogens.• Developed a pilot project proposal that became one of three finalists in the Research Director’s Innovation Challenge contest. • Introduced team building and new employee mentoring initiatives that enhanced the camaraderie and the culture of BASF Plant Science and decreased the assimilation time of new employees. -Founded and contributed to a New Employee Committee for more than 3 years. -Founded, staffed and chaired the Employee Newsletter Committee for over 7 years.• Workshops Include: - Introduction to TYPE in Organizations: Consulting Psychologists Press Inc - Critical Thinking and Project Management: Business Process Incorporated - Building a High Performance Team: Gigi Gardner Consulting LLC Show less

  • Scientist - Protein Analytics and Regulatory

    Nov 2012 - Sept 2016

  • Scientist - Crop Physiology and Phenotyping

    Jul 2001 - Oct 2012
• 

  BioAgilytix

  Apr 2017 - Aug 2019

  QC Specialist

  Review data and reports for a pharmaceutical CRO to ensure full GLP, GMP, and GCP compliance.

• 

  North Carolina State University Department of Biomedical Engineering

  Aug 2019 - now

  Laboratory Manager