Federica Poletti

Experience

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  University of Milan

  Jan 2010 - Mar 2011

  Università degli Studi di Milano - Cattaneo Lab

  Development of a protocol for the differentiation of human embryonic stem cells towards a neuronal fate.Maintenance of cell cultures, in particular human embryonic cell cultures and their feeders (murine embryonic fibroblasts). Extraction of RNA and DNA from tissue and cell samples. Gene expression analysis by RT-PCR and PCR. Immunofluorescence analysis.FACS analysis and good knowledge of confocal microscopy.

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  IFOM

  Jul 2011 - Aug 2017

  Research Laboratory Technician

  Maintenance of normal, primary, tumoral, stem cells, induced pluripotent stem cells (human, murine) cell cultures. Cell bank authentication and quality control with molecular biology and biochemical techniques (STR profile, mycoplasma detection). Cell engineering and gene expression analysis. Molecular cytogenetic techniques. Training to work in sterility in BSL1 and BSL2 rooms

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  ADIENNE Pharma & Biotech

  Aug 2017 - Mar 2019

  GMP Manufacturing and Development Technician - Cell Process

  - Perform manufacturing operation in clean room with hybridoma cell lines, following GMP requirements- Develop a cell based QC test with FACS

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  Axxam

  Apr 2019 - Dec 2024

  - Managment and coordination of people in Cell Factory Unit with activity of cell culture and solutions preparation - Responsible to improve new business strategies for cell engineering, primary cell lines and OMICS projects- Responsible for all cell factory managerial tasks including ordering, ensuring stock maintenance, administering cell lines repositories - Responsible Database Axxam Cell Lines- Responsible of coordination cell lines provisions for Assay Development Unit, HTS Unit, Compound profiling Unit , High Content Immunofluorescence Unit, and Ephys Unit- Studying new cell lines income in Axxam both from clients and internal unit to guarantee MB and WB cells generation with respecting QC - Supervision on preparation of solutions - Supervision and coordination internal references for LN2 Big container- Collaboration with Business department like Proposal Specialist- Collaboration with Legal department like FTO Specialist for Cell Lines- Collaboration with Purchasing and Logistic to improve order and quality of material useful for Cell Culture- Development of Standard Operating Procedures (SOP) in collaboration with QA,Facility Manager and HSE- Implementation of Unit assets selection of skilled people Show less - Mammalian cell culture handling: seeding, growing, thawing and freezing technics and cell culture media preparation- Insect cell culture handling: seeding, growing, thawing and freezing technics - Cell banking for the Axxam Cell lines/Assays repository of MB and WB with respect of QC- Cell engineering and gene expression analysis- Cell culture expansion and seeding for ephys analysis- Cell handling in large scale format and plating in 384 MTP for HTS purpose compound profiling and hit to lead purpose, by using highly automated dispending devices. (Multidrop and Matrix Well Mate)- Cell culture differentiation and plating in 384 MTP for HTS.- Preparation of solution and buffer for the whole Company Show less

  • Unit Manager Cell Factory - Molecular Cellular Biology Group

    Jan 2022 - Dec 2024

  • Senior Cell Biology Research Technician - Cell Factory Unit

    Apr 2019 - Dec 2021