Yashoda Subedi

Experience

• 

  Kathmandu model hospital

  Jun 2017 - Feb 2018

  Assistant Microbiologist (Intern)

  • Performed initial client assessment and analysis to begin the research process.• Handled microbiological equipment as a procedure and ordered research supplies.• Collected and processed specimens from more than 50 patients on daily basis.• Followed good laboratory and good clinical practice guidelines.• Performed ELISA, radioimmunoassay, and PCR to identify infectious agents and diseases. • Maintained lab cleanliness and safety.• Cooperated and communicated effectively with physicians to ensure client satisfaction and compliance with set standards.• Provided patient diagnoses under strict time constraints.• Interpreted research findings and summarized data into reports.• Diagnosed various common to highly infectious diseases.• Managed overall laboratory functions.• Helped and assisted colleagues and supervisors with their projects. Show less

• 

  Kansas State University

  Jan 2020 - Dec 2021

  • Performed research-related experiments in the food safety and defense laboratory to develop commercially applied antimicrobial intervention processes during wheat milling using various laboratory tools and techniques.• Performed experiments to validate commercially applied antimicrobial intervention process to control foodborne pathogenic bacteria during meat processing.• Used bacterial culture method, centrifugation, ELISA, moisture analyzer, and pH meter to conduct experiments. • Supervised undergraduate food microbiology lab workers and trained them on basic microbiology techniques, laboratory biosafety and biosecurity protocols, and general laboratory maintenance.• Recorded data, analyzed test results, and kept meticulous reports.• Developed and followed Standard Operating Procedures. • Maintained laboratory clean by following good laboratory practices. • Performed a literature review of more than 6200 articles for data collection, data synthesis, and statistical analysis to conduct a systematic review and meta-analysis. • Contributed to report writing and presentations. Show less • Assisted professor in preparing laboratory for experiments.• Ordered laboratory supplies as needed and kept record. • Supervised a group of 12-23 student while conducting laboratory experiments. • Assessed the abilities, strengths, and weaknesses of students and supported them as needed.• Worked with students individually to help them overcome specific learning challenges• Instructed and taught students according to the syllabus.• Checked students’ assignments on regular basis and provided feedback. Show less

  • Graduate Research Assistant

    Aug 2019 - Dec 2021

  • Graduate Teaching Assistant

    Jan 2020 - Dec 2020
• 

  KANSAS STATE VETERINARY DIAGNOSTIC LABORATORY

  Mar 2022 - Nov 2022

  Research Assistant at Next-generation Sequencing
• 

  University of Rochester Medical Center

  Dec 2022 - now

  Research Technical Associate

  - Leading stem cell culture assays independently to develop various cell lines, such as retinal endothelial cells and retinal organoids.- Cultivating and managing mammalian cell lines for ongoing protein expression studies.- Conducting genotyping of human and pig cell lines to contribute valuable insights to research goals.- Isolating RNA from human endothelial cells for PCR and sequencing, showcasing a strong grasp of molecular biology techniques.- Independently processing mice tissue samples, isolating and amplifying DNA, and executing genotyping procedures.- Playing a pivotal role in the colony management of mice, supporting the success of vital research projects.- Conducting immunohistochemistry and immunocytochemistry assays with precision and autonomy.- Contributing expertise to western blotting assays, aiding in the identification of target proteins.- Diligently collecting, recording, and analyzing results from ongoing research experiments.- Organizing and preparing results for presentation at routine laboratory meetings, demonstrating effective communication skills.- Maintaining and managing detailed experimental records, regularly sharing findings with the principal investigator and team members.- Proactively searching for relevant literature to troubleshoot technical challenges in research projects.- Crafting and updating standard operating procedures to tailor approaches to the unique needs of research projects.- Keeping the laboratory reagent inventory current and well-organized.- Ensuring strict compliance with quality and regulatory policies, actively participating in SOP maintenance, and contributing to quality records for equipment.- Coordinating the seamless ordering of laboratory supplies and equipment to support uninterrupted operations. Show less