Stefanie Brizuela

Experience

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  Mathematics, Engineering, Science Achievement (MESA) program

  Oct 2015 - Jul 2020

  Scholar

  -attended conferences, completed volunteer hours, and have built networks with professors and faculty.-allowed me to mature my social skills through interactive expectations needed to develop networks involving labs at UCSC.

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  United States Student Association

  Jul 2016 - Jul 2017

  UCSC Delegate

  -collaborated with members of the organization to create strategic methods that would result in making higher education more affordable and accessible on campus, state, and federal levels.-contributed ideas in delegation meetings designed to partition the labor in amending the USSA's constitution and aim for mitigating ambiguity.-attended the national conference held on an online platform to finalize the proposed and improved amendments.

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  Mathematics, Engineering, Science Achievement (MESA) program Student Leadership Conference

  Oct 2016 - Oct 2016

  Attendee
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  University of California, Santa Cruz

  Jun 2017 - Jul 2020

  Principal Investigator: Dr. Angela N. BrooksMentor: Brandon Saint-JohnCurrent Project: Attenuation of chromatin remodelers and transcription factors and its association with alternative splicing eventsPrevious Project: Loss of expression of SMARCA4 in lung cell lines and its association in alternative splicing events-mapped mouse lung cell line RNA-seq data via HISAT2 to appropriate genome-identified and classified splicing events via juncBASE analysis program with processed RNA-seq reads as inputPrevious Project: contrasting DNA methylation detection between nanopore sequencing and bisulfite sequencing to potentially use nanopore for detecting long-range associations with exon inclusion-analyzed MCF10A (non-tumorigenic breast cell line) RNA-seq short reads from bisulfite sequencing by mapping reads to genome reference using HISAT2 and observing methylation calls on sequence using Bismark-identified and classified splicing events via juncBASE, a splicing analysis program developed by Dr. Brooks, from mapped RNA-seq reads and followed with BedTools to intersect RNA-seq methylation calls for association Show less

  • Undergraduate Genomics Researcher

    Jun 2017 - Jul 2020

  • Session co-leader/Peer mentor

    Sept 2018 - Dec 2018

  • Student Ambassador

    Feb 2016 - Feb 2018

  • NIH-Big Data 2 Knowledge (BD2K) Summer Undergraduate Program Scholar

    Jun 2017 - Aug 2017
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  National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)

  Jun 2018 - Aug 2018

  Advanced Student Biological Sciences Technician

  Principal investigator: Dr. Elissa LeiNIH UGSP mentor: Dr. Ezequiel NazerSummer Project: Using dCas9 to attenuate transcription and its effect on active promoters in Drosophila melanogaster-produced dCas9 primers/protein via transformation of bacterial cells and transfection in embryonic D. melanogaster-analyzed efficiency of dCas9 recruitment of promoters with RNA/DNA/protein extraction via mini prep, followed with a western blot and chromatin immunoprecipitation, and nuclear immunofluorescence-observed transcriptional suppression by designing cDNA primers on PrimerPlus for D. melanogaster to perform quantitative RT-PCR Show less

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  National Cancer Institute (NCI)

  Jun 2019 - Aug 2019

  Advanced Student Biological Sciences Technician

  Principal Investigator: Dr. Dan LarsonSummer Project: Mutation in splicing factor U2AF1 governs RNA processing- compiled python scripts with the goal of quantifying ribosomal foot printing coverage with RNA-binding protein sites at intronic, exotic, coding, and untranslated regions; statistical cross-correlation will implicate if U2AF1 binding is affecting translation

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  Minaris Regenerative Medicine

  Aug 2020 - Sept 2023

  Cell and Gene Therapy Specialist
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  National Human Genome Research Institute (NHGRI)

  Apr 2021 - now

  Special Volunteer

  Principal Investigator: Dr. Laura ElnitskiProject: I am researching acute myeloid leukemia (LAML) and how LAML methylation signatures influence gene expression leading to cancer progression. I have developed a series of python and R scripts to stratify LAML TCGA samples based on methylation patterns that have allowed our lab to visually observe differences in isoform expression.Experience: I was a post baccalaureate scholar for two years before I transitioned into a special volunteer role. Show less