Karthik Ganganna

Experience

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  Bio-Invent Lab, Siddaganga Institute of Technology.

  Jan 2019 - Jun 2019

  Researcher

  ● Prepared lipid nanoparticle drug delivery system as batches with detailed documentation of each batch.● Improved production protocols for efficient drug loading through reduced lamellarity.● Optimized size of liposomes within nano size range using extrusion, used DLS to analyze size and visualized using Cryo-TEM.● Developed Concentration plots for mitocurcumin drug release kinetics and sample loading using HPLC.● Assisted in MSC cell culture and PBMC isolation for creating membrane vesicles Show less

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  Lunds universitet

  Aug 2019 - Dec 2021

  ● Led the development of western blot protocol for Ser/Thr kinase, identified two kinase proteins, involved with pneumococcal energy pathways.● Developed depolarization assays to check effect of ions on pneumococcal death. ● Successfully assisted in troubleshooting of fluorescent plate reader to deliver results on time along with accurate reagent documentation.● Performed routine tasks with GLP, assisted in autoclaving glassware, formulating media and transportation of media aseptically between departments.● Analysed data using GraphPad Prism, developed figures for assays, providing statistical analysis for the data. Show less ● Identification of Infectious Gram-negative bacteria using Uri-Select chromogenic plates.● Testing of Total Coliforms in minced meat.● Transcript quantification using reverse transcriptase PCR and real time PCR (qRT-PCR). ● Site Directed Mutagenesis, Purification and Characterization of GFP variants in E.coli.● Role of various genes in degradation of the cryptic protein GudB. ● Microbial Enrichment to isolate aerobic spore forming bacteria from soil. ● Developed a Business Model Canvas - Greenify, focus on improving water quality and production of algal fertilizers. Show less

  • Clinical Microbiologist

    Jan 2021 - Dec 2021

  • Molecular Biologist

    Aug 2020 - Jan 2021

  • International Mentor

    Aug 2020 - Dec 2020

  • Microbiology - Junior Researcher

    Aug 2019 - Aug 2020
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  NICE: the Network for Intercultural Competence to facilitate Entrepreneurship

  Feb 2020 - Jun 2020

  Researcher
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  NICE: the Network for Intercultural Competence to facilitate Entrepreneurship

  Feb 2021 - Jul 2021

  Facilitator

  Successfully managed a 5-person team of students in creating awareness of 'Fairtrade' in cocoa production.Conducted surveys, provided supportive feedback to prepare a business plan for sustainable and fair cocoa production.

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  Common Purpose

  Jul 2021 - Aug 2021

  Volunteer

  Micro Internship - Worked on a sustainable solution to revive coral reefs in Lang Tengah Islands, Malaysia via Lang Tengah Turtle Watch organization and Common purpose. Earned a Leading Change badge

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  AstraZeneca

  Feb 2022 - Jul 2022

  Lab Scientist

  QRIOS Consultant ● Handled human biological samples, produced high quality results within a 24-hour turnaround time to support a safe and healthy working environment.● Operated Liquid handling robots, assisted in troubleshooting, training users, assisted with instrument and inventory maintenance.● Delivered over 150,000 PCR sample results, trained in LIMS, writing SOPs, Lab cleaning and reagent batch documentation in an ISO/IEC 17025 accredited lab.● Experienced with Genedata, recording data on BioELN and in contamination control assays. Show less

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  TATAA Biocenter

  Oct 2022 - now

  Laboratory Scientist