Ramanan Devaraj

Experience

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  National Institute of Oceanography

  Dec 2009 - May 2010

  Project Trainee

  M.Sc Dissertation work entitled as “Interaction of autochthonous flora from Afanasiy Nikitin Seamount on Ferromanganese crust and the incidence of plasmids in selected bacterial isolates involved”

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  Institute Of Microbial Technology

  Jul 2011 - Dec 2011

  Project Assistant

  Functional Characterization of WhiB5/Rvo22c and WhiB6/Rv3682c Protein of Mycobacterium Tuberculosis H37Rv* Prepared protein expression constructs and optimized IPTG induced overexpression of recombinant proteins in 5 to 10-liter bacterial culture.* Acquired hands-on skills in protein expression, purification by AKTA-FPLC followed by SDS-PAGE and WB.

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  Postgraduate Institute of Medical Education and Research

  Jan 2012 - May 2013

  Junior Research Fellow

  Genotype-phenotype analysis of Connexin 30, SLC26A4 and mitochondrial genes in Non-syndromic Hearing Loss (NSHL) patients * Optimized PCR method with 24 sets of primers to amplify whole mitochondrial genome of NSHL patients-derived blood samples.* Compiled DNA sequencing data, performed mutational analysis with Codon-Code Aligner software* The resultant data of 50 Non-syndromic Hearing Loss Patient's whole mitochondrial genome sequences and 9 novel mitochondrial sequences were submitted in GenBank. Show less

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  Academia Sinica, Taiwan

  May 2012 - Jun 2012

  Summer Intern at IMB

  TIGP-International Internship Program Cellular Localization of Minor Degradosome Components in E. coli

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  Institute of Molecular Biology, Academia Sinica.

  Jan 2014 - Aug 2015

  Doctoral Researcher

  Telomerase regulation in Short Telomeres Rapid Elongation* Established in vitro model system by stably expressing TPP1-∆RD and OB fold domain S111A and TEL patch mutants in cancer cell lines and studied telomerase regulation for rapid telomere elongation using Telomere Restriction Fragment (TRF) analysis.* Mastered in molecular cloning, mutant constructs by site-directed mutagenesis, generating stable cell lines, Telomere Restriction Fragment (TRF) analysis to measure telomere length. Show less

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  Genomics Research Center, Academia Sinica

  Jan 2016 - Oct 2022

  Doctoral Researcher

  PhD in Molecular and Cell BiologyThesis Title:Mechanistic Study of N-α-acetyltransferase 10 Protein in Preventing Dystocia(Manuscript in preparation)* Mastered in performing timed-pregnant, C-section, preterm birth experiments to study the "labor".* Using an ELISA, LC-MS and RT-qPCR, studied the hormonal regulation and ovarian P4 withdrawal pathways in the late gestation mice (18.5 and 19.5 d.p.c).* Using a cell-based in vitro contraction assay (3D culture), studied the uterus contraction from primary and hTERT immortalized human myometrial cells.* Using confocal, live cell imaging, In vitro acetyltransferase assays and LC-MS based methods, studied actin cytoskeleton remodeling and N-terminal acetylated substrates.Additional project:Naa10p Inhibits Beige Adipocyte-Mediated Thermogenesis through N-α-acetylation of Pgc1α.(Lee et al., and Juan, Mol. Cell. 2019, * as co-author)* Established LC-MS based In vitro acetyltransferase assay (AT assay) using recombinant purified hNaa10p.* This method has helped us to identify the key mechanistic step - N-α-acetylation status of PGC1α, PPARγ. Show less