Alessandra Gallo

Education

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  Instiut Curie, Centre de Recherche, Paris, France – U934/UMR3215; Sapienza University of Rome, Italy

  2013 - 2014

  Stage- Leonardo Da Vinci Program-Unipharma Graduates 9 Neurobiology and Neurosciences

  The research project concerns the analysis of posttranslational modifications in axonal transport control using zebrafish embryos as a model.

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  Université de Paris, ENP, Ecole des Neurosciences de Paris

  2014 - 2019

  PhD Neuroscience

  Activities and Societies: Institut de Psychiatrie et Neurosciences de Paris-INSERM U1266 Membrane Traffic in Healthy & Diseased Brain, Equipe GALLI Title: Role of ER-plasma membrane contact sites in neuronal developmentAxons and dendrites are long and ramified neurites that need intense plasma membrane (PM) expansion during nervous system development. Neurite growth depends on non-fusogenic Sec22b–Stx1 SNARE complexes at endoplasmic reticulum (ER)-PM contacts. Here we show that Sec22b interacts with the ER lipid transfer proteins Extended-Synaptotagmins (E-Syts). Overexpression of E-Syts stabilizes Sec22b-Stx1 association, whereas… Show more Title: Role of ER-plasma membrane contact sites in neuronal developmentAxons and dendrites are long and ramified neurites that need intense plasma membrane (PM) expansion during nervous system development. Neurite growth depends on non-fusogenic Sec22b–Stx1 SNARE complexes at endoplasmic reticulum (ER)-PM contacts. Here we show that Sec22b interacts with the ER lipid transfer proteins Extended-Synaptotagmins (E-Syts). Overexpression of E-Syts stabilizes Sec22b-Stx1 association, whereas silencing of E-Syts has the opposite effect. Overexpression of wild-type E-Syt2, but not mutants unable to transfer lipids, increase the formation of axonal filopodia and ramification in developing neurons. This effect is inhibited by a clostridial neurotoxin cleaving Stx1, expression of Sec22b Longin domain and a Sec22b mutant with extended linker between SNARE and transmembrane domains. We conclude that Sec22b-Stx1 ER-PM contact sites contribute to PM expansion by interacting with E-Syts. Show less

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  Sapienza Università di Roma

  2007 - 2010

  Laurea triennale in scienze biologiche 110 cum laude

  Thesis Title: The role of nitric oxide in central e peripheral nervous system

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  Università degli Studi di Roma 'La Sapienza'

  2010 - 2012

  Laurea Magistrale LM in Biologia Applicata alla ricerca biomedica Neurobiology and Neurosciences 110 cum laude

  Thesis Title: Lack of dystrophin alters NGF-dependent neurite outgrowth: a study on wild-type and mdx mice