Nikolas Mathioudakis, Ph.D

Experience

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  IMBB-FORTH

  Sept 2005 - Sept 2007

  Research Intern

  Diploma thesis:"Expression, purification and biochemical characterization using refolding techniques of the C-terminus of the protein HrcU from the Type Three Secretion System of the plant pathogen Pseudomonas syringae pv. Phaseolicola (Psph)".Additional Projects(funded practical training): " Co-expression and purification of proteins HrpG and HrpV from the type III secretion system of the plant pathogen Pseudomonas syringae pv. Phaseolicola (Psph)""Expression, purification and crystallization of mutant protein HrcQb-C Ile85-Trp of the plant pathogen Pseudomonas syringae pv. Phaseolicola (Psph)""Cloning of the hrpD gene and optimization of the solubilization of the HrpD protein from the Type Three Secretion System of the plant pathogen Pseudomonas syringae pv. Phaseolicola (Psph)"X-ray CrystallographyBiochemistryMolecular Biology Show less

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  IMBB-FORTH (Prof. M.Kokkinidis laboratory)

  Jan 2008 - Jul 2008

  Master student

  Training in a research project entitled "Data processing and function of X-ray generator - an in theory approach"Learning basic commands on LINUXCollection of crystallographic dataData processing

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  European Molecular Biology Laboratory

  Sept 2008 - Sept 2012

  Pre-Doctoral Fellow

  Dissertation title: "Structural and functional studies on the piRNA pathway component TDRD1"Description:Piwi-interacting RNAs (piRNAs) are small non-coding RNAs expressed in the germ line of animals. They associate with Argonaute proteins of the PIWI subfamily, forming ribonucleoprotein complexes that are involved in maintaining genome integrity. The N-terminal region of some PIWI proteins contains symmetrically dimethylated arginines. This symmetrical dimethylation is thought to be responsible for the recruitment of Tudor domain-containing proteins (TDRDs), which might serve as platforms mediating interactions between various proteins in the piRNA pathway. We measured the binding affinity of the four individual extended Tudor domains (TDs) of murine TDRD1 protein for three different methyl-arginine containing peptides from murine PIWI protein Mili. The results show a preference of TD2 and TD3 for consecutive Mili peptides whereas TD4 and TD1 have respectively lower and very weak affinity for any peptide. These observations were confirmed by pull-down experiments with endogenous PIWI and PIWI-associated proteins. The affinity of TD1 for methyl-arginine peptides can be restored by a single point mutation in the aromatic cage back to the consensus sequence. The crystal structure of TD3 bound to a methylated Mili peptide shows an unexpected, non-canonical orientation of the bound peptide and of the methylated arginine side-chain in the aromatic cage. Finally, the SAXS model of the four tandem Tudor domains of TDRD1 reveals a flexible, elongated shape of the protein. Overall the results show that TDRD1 can accommodate different peptides from different proteins and therefore act as a scaffold protein in the piRNA pathway.Structural BiologyX-ray CrystallographyProtein Biochemistry-BiophysicsMolecular Biology Show less

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  NCSR "DEMOKRITOS"

  Sept 2013 - Jan 2015

  Postdoctoral Fellow

  Dr. Efstratios Stratikos laboratoryhttps://sites.google.com/site/stratikos/Research Projects:ER Aminopeptidases and the immune response: Structural and functional characterization of antigen selection and processing by ERAP1 and ERAP2 proteins.Research Areas: Immunology, Protein Chemistry, Biochemistry

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  IQVIA

  Jan 2015 - Nov 2018

  Key Responsibilities:• Provide in-house support to the clinical project team.• Trial submissions to RA, CEC and IRB/IEC.• Tracking clinical documents and SAEs.• Setting up and managing trial files.• Trial supplies (drug, equipment, etc.).• Central laboratory, couriers, etc.• Coordinating data flow in-house.• Obtain site related trial documents and review them for completeness and accuracy and SOP and regulatory compliance.General Activities:• Awareness of knowledge of applicable clinical research regulatory requirements; i.e., Good Clinical Practice (GCP) and International Conference on Harmonization (ICH) guidelines.• Represent the company and the organization adequately to the customers and other externalparties.• Maintain adequate communication with relevant parties within and outside the company.• Comply with ICH-GCP and all applicable local laws and regulations.• Actively follow trainings to ensure adequate qualification for performing job-related tasks.• Regularly update information in applicable systems and templates. Show less

  • Clinical Research Associate

    May 2016 - Nov 2018

  • Senior ISS / Local Regulatory Start-Up Coordinator

    Mar 2016 - May 2016

  • ISS/Local Regulatory Start-Up Coordinator

    May 2015 - Mar 2016

  • Clinical Trial Assistant

    Jan 2015 - May 2015
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  AstraZeneca

  Nov 2018 - now

  Respiratory Area

  • Medical Manager Rare Diseases

    Aug 2022 - now

  • Field Medical Manager Biologics, Respiratory & Immunology

    Jun 2021 - Jul 2022

  • Medical Science Liaison

    Nov 2018 - Jun 2021