Maike Getwan

Experience

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  University of Hohenheim

  Dec 2009 - Apr 2015

  PhD student

  I was part of the Martin Blum group, which focused on left-right-axis development of amphibian and mammal embryos but also see urchins and chickens. my project:My PhD thesis concentrated on the establishment of left-right asymmetry during early development of Xenopus. my goal:To find out how the first asymmetrically expressed gene Dand5 (or: Coco) is regulated (transcriptionally, post-transcriptionally, translationally).most important methods:- analyse gene interactions with rescue-experiments and additive effect of RNA/DNA/morpholino oligonucleotides- miRNAs (injection (gain and loss of function), detection with in situ hybridisation)- working with Xenopus laevis (in vitro fertization, cultivation)- DNA- handling (DNA (PCR, cloning, cDNA synthesis/ RT-PCR, extraction, detection via gel electrophoresis, sequencing) - RNA-handling (total RNA extraction/ isolation, in situ hybridization, synthesis, injection into embryos)- detection/ staining of protein (whole embryos/ organs and sections; preparation of tissue (fixation); staining for multiple proteins at once; testing of new antibodies; DAPI; phalloidin)- morpholino oligonucleotides- phenotype analysis- confocal and stereo-microscopy- microinjection and micromanipulationfurther:- graphical programs (Adobe, ImageJ)- presentation of results (international conference, internally)- supervision of student courses as well as bachelor, master and doctoral students during their thesis' in the labpublications:DOI: 10.1038/s41467-021-25464-zDOI: 10.1016/j.cub.2010.02.061 Show less

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  Medical University of Freiburg

  Oct 2015 - Sept 2019

  Scientist (postdoctoral researcher)

  Member of the Soeren Lienkamp group focusing on kidney research (nephrology, nephropathies)my project: A rare group of diseases called "Short Rib Thoracic Dysplasia" (SRTD) belonging to skeletal ciliopathies/ chondrodysplasia. They are linked to nephropathies and have their origin in mutations of cilia genes.my goal: identification and characterization of novel SRTD associated genes most important methods: - phenotype analysis of Xenopus laevis and Xenopus tropicalis crispants (embryos and adults)- microinjections- stainings and detections of mRNA and proteins (in situ hybridisation, immunhistochemistry)- microscopy (LSM, confocal)- cloning- CRISPR/Cas9further:- Statistical analysis (GraphPad Prism, ImageJ)- Graphical programs (Adobe, Affinity, ImageJ)- Write scientific manuscripts- Supervision of PhD students and medical studentspublication: DOI: 10.1073/pnas.2106770118 Show less

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  University of Zurich

  Oct 2019 - Sept 2022

  Scientist (postdoctoral researcher)

  Continuation of the work on skeletal ciliopathy genes from Freiburg in the lab of Soeren Lienkamp in Zurichnew methods:- histology (Technovit, stainings)- microCT with froglets- Creating, breeding and analysis of transgene Xenopus CRISPR/Cas9-lines- Optimization, adapting and generating of genotyping methods for Xenopus CRISPR/Cas9 embryos and adults (DNA extraction with hundreds of embryos, genotyping)

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  Ariya Bio

  Sept 2023 - Dec 2023

  Scientist

  Ariya Bio developes next-generation gene therapies for inherited blood diseases with CRISPR/Cas9Techniques and goals:• Cellculture with non-adherent cell line HUDEP2 and hPSC in a BSL-2 environment• Knock-in with CRISPR/Cas9 (design of constructs, RNP nucleofection of cells)• Increasing knockin efficency of CRISPR/Cas9• Multiplexing with CRISPR/Cas9 (combination of kockout and knockin)• Gene editing efficency testing with NGS (Next Generation Sequencing) • HPLC• qPCR• Flow Cytometry Show less

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  Cytosurge AG

  Jan 2024 - Aug 2024

  R&D Scientist Biology

  Cytosurge AG offers a particularly gentle way of manipulating cell lines: By injecting individual cells, they are edited at the gene level. Areas of responsibility: • Creating and optimizing methods and protocols/work instructions • Gene editing of cell lines (CRISPR/Cas9) • Optimization of CRISPR/Cas9 knockins • Cell culture with adheren cell lines (C2C12, C2C12 with GFP, HEK, HeLa, U2OS): • Maintenance of cell lines (trypsin treatment, keeping in culture, different types of cell number determination) • Cryopreservation (freezing and thawing of cell lines) • Single cell seeding • generate and maintain monoclonal cell lines • Optimizing the handling of cells for injections (e.g. plating) • PCR optimization for genotyping with NGS• Working with online laboratory journals • Presenting results to internal steakholders Show less