Talk to sales Try for Free
Laetitia ANDRIQUE

Laetitia ANDRIQUE

Post-doctoral researcher

Followers of Laetitia ANDRIQUE215 followers
location of Laetitia ANDRIQUEGreater Bordeaux Metropolitan Area

Connect with Laetitia ANDRIQUE to Send Message

Connect

Connect with Laetitia ANDRIQUE to Send Message

Connect

  • Timeline

  • About me

    Ingénieur de Recherche CNRS, Responsable Scientifique et Technique de la Plateforme VoxCell, Production d'organoïdes 3D

  • Education

    • Université de Poitiers

      2003 - 2008
      Doctorat Biologie et Génétique Cellulaires et Moléculaires - Biotechnologies

      ABSTRACTp14ARF is a tumor suppressor gene which inhibits cell proliferation by the ARF/MDM2/P53 pathway.When p53 is deleted, ARF is still able to limit cell proliferation. In this p53 independent context, I showed that p14ARF specifically interacts with rDNA promoter, inhibits rRNA transcription and ribosome biogenesis via UBF1 and E2F1.RESULTSANDRIQUE L et al, Cell Signalling 2012Ayrault O, ANDRIQUE L et al, Oncogene 2006Ayrault O, ANDRIQUE L et al… Voir plus ABSTRACTp14ARF is a tumor suppressor gene which inhibits cell proliferation by the ARF/MDM2/P53 pathway.When p53 is deleted, ARF is still able to limit cell proliferation. In this p53 independent context, I showed that p14ARF specifically interacts with rDNA promoter, inhibits rRNA transcription and ribosome biogenesis via UBF1 and E2F1.RESULTSANDRIQUE L et al, Cell Signalling 2012Ayrault O, ANDRIQUE L et al, Oncogene 2006Ayrault O, ANDRIQUE L et al, Medecine/Science 2006ANDRIQUE L et al, Exp Cell Res 2006ANDRIQUE L, Ayrault O et al, Oncogene 2005Ayrault O, ANDRIQUE L et al, Oncogene 2004 Voir moins

    • Université de Poitiers

      1998 - 2003
      Master 2 (M2) Biologie et génétique cellulaires et moléculaires

  • Experience

    • Inserm

      Jan 2009 - Jan 2012
      Post-doctoral researcher

      PROJECT MANAGEMENTIs FoxA1 a driver gene of luminal breast cancer ?1/ Methodology 1: Engineering and cloning of viral vectors (IDLV, lentivirus) to generate targeted integration of a rapporter gene (GFP/Puro resistance) in frame with FoxA1 gene2/ Methodology 2 : Lentiviral transduction in order to overexpress FoxA1 in mammary primary cells (BPEC) or in pseudo-normal cells (MCF10A). Mammospheres, colony assay (E-CFC) and phenotype analysis before xenograft in mammary fat pad of immunodeficient miceRESPONSIBILITIES1/ P2 and P3 laboratories management: inventory and stock management, SOP (standard operating procedures), training of external users2/ Management of staff and students3/ Work in a A2 animal housing facility Voir moins

    • SIRIC BRIO - Bordeaux Recherche Intégrée en Oncologie

      Jan 2012 - Jan 2015
      Scientist - Engineer

      PROJECTS MANAGEMENTCutaneous lymphoma Mouse models (PDX, CLX)Telomerase functions in cutaneous lymphomasRESPONSIBILITIES- Scientific publication writing- Grant and annual report writing- Management of staff and studentsTECHNOLOGY- primary cell culture (from blood or skin biopsy)- lentivirus: from cloning to transduction- functionnal cell study : migration, proliferation, tumorigenicity- in vivo proof of concept : establishment of efficient xenograft mouse models, necropsy- histology : IHC, HES, ImmunostainingRESULTS1/ Publication, 2nd author, Oncotarget, Sept 2016:"Molecular alterations and tumor suppressive function of the DUSP22 gene in peripheral T-cell lymphoma subtypes"2/ Publication, 1rst author, Am J Pathol, May 2016:"Intrahepatic Xenograft of Cutaneous T-Cell Lymphoma Cell Lines: A Useful Model for Rapid Biological and Therapeutic Evaluation"3/ Patent of invention, co-inventor, submitted FR15/61731, dec 2015 (mouse model of cutaneous lymphomas)4/ Publication: 2nd author, Blood, 2014:"Telomerase functions beyond telomere maintenance in primary cutaneous T-cell lymphoma"5/ Publication: Pham-Ledard L, Carlotti M, Andrique L et al, Modern Pathol, 2014 Voir moins

    • INSERM U1029/LP2N Institut d'Optique d'Aquitaine

      Jan 2016 - Nov 2020
      Scientist

      PROJECT MANAGEMENTVascular Tissue engineering/Bioprinting --​> a new model of guided self-organization allowing rapid formation of functional artificial vesselsTECHNOLOGY- bioprinting- primary cell culture- lentivirus from cloning to cell transduction- cell encapsulation in alginate microcapsules/microtubes using a microfluidic device- 3D immunostaining and analysis (confocal/videomicroscopy)- 2D/3D FIJI image analysisRESULTS- 03/2017 : patent of invention in vascular tissue engineering "Microfibre cellulaire creuse et procédé de fabrication d’une telle microfibre cellulaire creuse"​ (request number FR1751941) - co-inventor- 02/2017 : publication "All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide"​ (2017, Scientific Reports) - co-1rst author: http://www.nature.com/articles/srep42378- 12/2016 : patent of invention "Chambre de culture et d'imagerie d'échantillons biologiques"​ (request number 16 63241) - co-inventor Voir moins

    • CNRS - Centre national de la recherche scientifique

      Nov 2020 - now
      Ingénieur de Recherche

  • Licenses & Certifications

    • Bonnes Pratiques en Recherche Clinique

      Dirc grand ouest
      Feb 2013

    • Spécialisation en chirurgie sur petit animal

      CNRS
      Feb 2013

    • Experimentation Animale Niveau I

      GRETA Loiret Centre
      Oct 2010