Laszlo Mozsik

Experience

• 

  Teva Pharmaceuticals

  Jun 2012 - Nov 2013

  Method development for the isolation of Mupirocin impurities with preparative HPLC Working in GMP systems: Tablet filling line operator, Manual tablet blister packing

  • Internship - Chemical Research and Development

    Jul 2013 - Nov 2013

  • Part time production line worker

    Jun 2012 - Jun 2013
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  University of Debrecen

  Feb 2014 - Aug 2014

  Internship - Department of Biochemical Engineering

  Examination of extra- and intracellular lactose catabolism in Penicillium chrysogenum

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  Teva Pharmaceuticals

  Jul 2014 - Aug 2014

  Internship - Biological Testing Department

  ELISA (enzyme-linked immunosorbent assay) tests using mammalian cell line cultures on the field of innovative drugs

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  Rijksuniversiteit Groningen

  Sept 2014 - Dec 2014

  Research Internship - Department of Molecular Microbiology

  Activity studies of amoxicillin forming Isopenicillin N synthase mutants

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  Rijksuniversiteit Groningen

  Mar 2015 - Sept 2015

  Master's Thesis - Department of Molecular Microbiology

  Development of a novel, orthogonal, synthetic, transcriptional control device for P. chrysogenum• Developed skills with modular DNA assemblies (Golden Gate, Modular Cloning, Gibson Assembly)• Designed synthetic transcriptional factor fusions and genetic circuits for transcriptional activation• Performed native gene overexpression and heterologous gene overexpression• Molecular microbiology techniques (PCR, protein fusions, transformation, RT-PCR expression analysis)

• 

  Rijksuniversiteit Groningen

  Nov 2016 - Apr 2021

  PhD Researcher

  Synthetic biology tools for transcriptional activation and regulation of biosynthetic gene clusters in filamentous fungi:-Modular synthetic biology toolkit for filamentous fungi-Synthetic control devices for gene regulation in Penicillium chrysogenum (rubens)-Genome editing in Penicillium chrysogenum (rubens) using Cas9 ribonucleoprotein particles-Utilization of CRISPRa/dCas9 for transcriptional regulation in filamentous fungiThe CRISPR/Cas9 system is a recent molecular tool that has revolutionized genome editing of all kinds of cells. In the CRISPR/Cas9 genome editing technology, the Cas9 endonuclease is guided by a single chimeric guide RNA to a specific locus, where it introduces a double-strand break in the DNA sequence that can be used for enhanced homology-directed repair. During the ALERT program, the aim is to employ the Cas9 protein to allow for RNA-guided transcriptional regulation. This technology will be used to upregulate and interrogate silent endogenous secondary metabolite genes in Penicillium and will be transferred to other fungi.• Collaborated on various projects related to genome editing, protein engineering, natural product identification via LC-MS, secondary-metabolite-free platform-strain engineering and anti-microbial compound discovery• Supervised and performed projects on genetic modifications, cultivations and downstream analysis• Designed experiments and genetic circuits for transcriptional regulation in filamentous fungi• Acquired skills in CRISPR/Cas9-based genome editing and transcriptional activation (CRISPRa)• Performed various downstream analysis through LC-MS, U-HPLC, prep-HPLC, qPCR, whole-genome seq. Show less

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  Universiteit Leiden

  Jul 2021 - Jul 2022

  Postdoctoral Researcher

  Technology development for novel strain engineering tools using a class 2 type V CRISPR (BEC) gene-editing technology for an Aspergillus-based, high protein yield production strain (CRO-based)

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  Dsm-firmenich

  Aug 2022 - now

  Scientist (Associate II) R&D