Chandra Boosani

Experience

• 

  Boys Town

  Sept 2005 - Sept 2012

  Research Associate

  • Isolated NC1 domain sequences from cDNA library and deposited domain sequences in NCBI GenBank.• Generated transgenic mice with inducible expression of anti-angiogenic proteins and conducted in-vivo tumor studies in transgenic mice. • Produced and purified recombinant antiangiogenic proteins in E. coli and Sf9 cells, and identified novel signaling mechanisms of COX2 inhibition by angiogenesis inhibitors.• Tested the anti-angiogenic effects of Hexastatin on MC57 sarcoma cells.

• 

  Creighton University School of Medicine

  Sept 2012 - Jun 2019

  • Designed and adopted a two guide RNA CRISPR-Cas9 system, for producing BAH1 domain deletion mutant mice. Developed engineered mutant cell lines using lentivirus to deliver CRISPR-Cas9 constructs. • Identified molecular, epigenetic and immunological mechanisms that promote cellular hyperplasia. • Performed co-culture studies to identify the functions of HDAC10 engineered T-cells in inducing proliferation and migration of vascular smooth muscle cells.• Was responsible for developing strategic planning, designing effective experimental approaches, evaluating research personnel and conducting lab meetings. Show less

  • Research Assistant Professor

    Jan 2018 - Jun 2019

  • Postdoctoral Researcher

    Sept 2012 - Dec 2017
• 

  Western University of Health Sciences

  Jul 2019 - Jun 2020

  Assistant Professor

  Development of a unique mouse model for in-stent restenosis. Production of mutant T-cells using CRISPR-Cas9 system. Evaluation of immune regulation, macrophage polarization and foam cell formation by epigenetic mediators.