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Ravi C. Dwivedi

Ravi c. dwivedi

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location of Ravi C. DwivediLondon, Ontario, Canada
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  • Timeline

  • About me

    Founder, Bio-Medical Proteomics | Clinical Diagnostics & Healthcare-IT Industry | Biotechnologist | Biomedical Engineer

  • Education

    • Indian institute of technology, bombay

      1997 - 1999
      Mtech biomedical engineering gpa: 3.58/4.00 (world education service-wes canadian equivalency)

      Computer Aided drug design for HIV-I protease inhibitors, molecular docking using INSIGHT-II, followed by chemical synthesis of optimized molecules at the department of chemistry IIT-Bombay, and their testing using x-ray crystallography at Bhabha Atomic Research Centre ( BARC-Bombay) India. Participated in “Business Plan Competition, EUREKA” at IIT Bombay (organized at International Level) in which our team was among top 5 finalists in the year 2001.Coordinator of the Marketing… Show more Computer Aided drug design for HIV-I protease inhibitors, molecular docking using INSIGHT-II, followed by chemical synthesis of optimized molecules at the department of chemistry IIT-Bombay, and their testing using x-ray crystallography at Bhabha Atomic Research Centre ( BARC-Bombay) India. Participated in “Business Plan Competition, EUREKA” at IIT Bombay (organized at International Level) in which our team was among top 5 finalists in the year 2001.Coordinator of the Marketing team of "Mood Indigo-99" (largest youth festival of India) at IIT, Bombay.Courses: Signals and Systems for Biomedical Engineering; Medical Imaging Physics; Biomaterials; Electrodiagnostics Therapy and Electrical Safety; Elements of Circuit and Instrumentation; Ergonomics; Medical Sensors; Physiological Measurement and Quantitative Physiology; Biomathematics; Clinical Physiology Show less

    • Himachal pradesh university

      1995 - 1997
      M.sc. biotechnology gpa 3.81/4.00 (world education service-wes canadian equivalency)

      Application of psychrophilic ß-galactosidase in the commercial utilization of whey and hydrolysis of lactose in milk. Molecular cloning of glycolytic genes from S. pombe into S. cerevisiae mutants blocked in the glycolytic pathway at TIFR (Tata Institute of Fundamental Research) Bombay.Courses: Biophysics & Instrumental Method; Biostatistics and Computer Science; Biochemical Engineering; Fermentation Technology; Biochemistry; Enzyme Technology; Microbiology and Cell Biology;… Show more Application of psychrophilic ß-galactosidase in the commercial utilization of whey and hydrolysis of lactose in milk. Molecular cloning of glycolytic genes from S. pombe into S. cerevisiae mutants blocked in the glycolytic pathway at TIFR (Tata Institute of Fundamental Research) Bombay.Courses: Biophysics & Instrumental Method; Biostatistics and Computer Science; Biochemical Engineering; Fermentation Technology; Biochemistry; Enzyme Technology; Microbiology and Cell Biology; Genetics and Molecular Biology; Recombinant DNA Technology; Immunology and Immunotechnology; Plant Biotechnology; Animal Biotechnology; Environmental Biotechnology; Biotechnology in Processing Industry; Physical chemistry; Inorganic chemistry; Organic chemistry. Show less

    • The university of göttingen

      2002 - 2006
      Doctor of philosophy (phd) protein identification and characterization using protein biochemistry and proteomics platforms

      Thesis: Proteomic and biochemical analysis of extracellular proteins and redox-enzymes from Trametes versicolor. Worked towards optimization and implementation of proteomic methods for the isolation and purification of oxidative enzymes from higher basidiomycetous fungi. High-quality cell wall preparation, in-gel detection of phenoloxidases, characterization of supernatant and cell wall-associated laccase, the release of cell wall-associated laccase, and extracellular proteome analysis of… Show more Thesis: Proteomic and biochemical analysis of extracellular proteins and redox-enzymes from Trametes versicolor. Worked towards optimization and implementation of proteomic methods for the isolation and purification of oxidative enzymes from higher basidiomycetous fungi. High-quality cell wall preparation, in-gel detection of phenoloxidases, characterization of supernatant and cell wall-associated laccase, the release of cell wall-associated laccase, and extracellular proteome analysis of Trametes versicolor were performed from the supernatant, hyphal sheath fractions.Participant data management for 6th VAAM-conference, Göttingen, Germany. Show less

  • Experience

    • Transasia bio-medicals ltd.

      Jan 2000 - Jun 2001
      Assistant product manager

      Product management and corporate strategy of Electrolyte analyzers for all over India (monitoring sales, competition, product specification development, marketing communications, inventory management etc.). Application training, installation, demonstration and sales support for Electrolyte analyzer, Blood cell counter, ELISA systems to pathologists, service engineers, sales staff and potential customers. Assessed market potential for the launch of new products, which included competitor assessment, marketing strategy analysis for new markets. Participated in clinical diagnostics conferences and business fairs. Show less

    • Megasoft

      Jun 2001 - Sept 2002
      Product specialist and domain expert (e-pharma and e-health)

      Domain expert for healthcare & pharmaceutical sector in terms of advising software development team on clinical trials and drug development processes including FDA regulations for 21CFR11. Enabled processes of software development, maintenance, and enhancement of Acceliant (India’s first web-based clinical trial solution for Enmed Inc.). Acceliant offers solutions for electronic documentation, image management, audit trail, role-based access control, electronic records and signatures (21CFR Part 11).Business development of Acceliant in the Asia Pacific region, which included product demonstration, training & support for pharmaceutical clients and met financial goals.Domain and product development support for Hospital Management System. Show less

    • Manitoba centre for proteomics & systems biology

      Dec 2006 - Jun 2012
      Postdoctoral fellow

      Absolute quantitation and validation of C19orf10 protein using selective reaction monitoring (SRM) in serum and synovial fluid samples of Rheumatoid Arthritis patients.Studied the immunomodulatory effect of anti-inflammatory therapies (Infliximab, Methotrexate) in Rheumatoid Arthritis using proteomics platform in collaboration with Schering-Plough and AstraZeneca. Proteomic analysis of genetically deficient Leptin patients receiving leptin-replacement therapy in collaboration with Department of Psychiatry & Behavioral Sciences, University of Miami, USA. Show less

    • University of manitoba

      Jul 2012 - May 2017
      Research associate

      Identification and validation of biomarkers for Acute Kidney Injury caused as a result of cardiac surgery (using high-throughput proteomics and systems biology platforms).Study immune cell migration by understanding the role of 1) global protein phosphorylation in GMFG knock-down T-cell migration using CEM cells, 2) protein palmitoylation of NK cells using click chemistry, 3) activity based serine hydrolase profiling (ABPP) of normal vs. lethal B-cells, 4) GTPase profiling of normal and CXCL12 stimulated primary T-cells and Jurkat cells.RESEARCH PLATFORMS- Mass-spectrometry (MS): Time of flight, Triple Quadrupole, LC-MS, SWATH, SRM/MRM- Chromatography: Agilent HPLC, Eksigent nanoflow, 2D-HPLC, FPLC- Bioinformatics and data analysis: Ingenuity Pathway Analysis (IPA), KEGG, PANTHER, SPSS- Protein and peptide labelling: SILAC, iTRAQ, label-free, isotopic labelled peptides - Post-translational modifications: protein phosphorylation, protein palmitoylation- Affinity purification: IgY-12 serum depletion, hexapeptide library, immunoprecipitation (IP)- Protein separation and detection: 2D-PAGE, IEF, cye dyes, western-blot, ELISA, zymography- Activity-based protein profiling: for active serine hydrolases, desthiobiotin GTPase enrichment - Rational drug-design: molecular modelling, energy minimization, docking and chemical synthesis- Molecular biology: DNA/RNA isolation, detection (southern/northern), PCR, electroporation- Cell biology: cell culture, confocal microscopy Show less

    • Bio-medical proteomics

      Jul 2017 - now
      Founder

      Bio-Medical Proteomics is a BioTech Consulting and Contract Research Company. We identify and validate novel proteins of diagnostic, therapeutic and preventative value. BMP specializes in providing consultancy, data analysis, R&D for proteomics-based initiatives. We use platforms of Biochemistry, Immunology, Analytical Chemistry, Bioinformatics and Systems Biology to help clients.OUR SOLUTIONS- Proteomics consulting- Omics data analysis- Protein identification & verification- Biomarker discovery and validation- Post-translational modification (phosphorylation)- Activity-based protein profiling- GTPase profiling Show less

  • Licenses & Certifications

    • Ingenuity pathway analysis (ipa) certification for molecular pathway analysis

      Ingenuity pathway analysis
      Jul 2010
      View certificate certificate

    • Trained by abs/mds sciex for qrtap-4000, protein pilot and qstar-elite.

      Jun 2009

  • Honors & Awards

    • Awarded to Ravi C. Dwivedi
      Department of Biotechnology scholarship Central Government of India Recipient of DBT (Department of Biotechnology) scholarship 1995-1997.
    • Awarded to Ravi C. Dwivedi
      Graduate Aptitude Test in Engineering fellowship Central Government of India Recipient of GATE (Graduate Aptitude Test in Engineering) fellowship 1998-1999.
    • Awarded to Ravi C. Dwivedi
      Lindeman Stiftung fellowship Georg-August-Universität, Göttingen, Germany Recipient of Lindeman Stiftung fellowship 2002-2006.

  • Volunteer Experience

    • Tree planting volunteer

      Issued by Tree Canada / Arbres Canada on Jan 2015
      Tree Canada / Arbres CanadaAssociated with Ravi C. Dwivedi

    • Youth for Literacy Campaign

      Issued by National Service Scheme on Jan 1992
      National Service SchemeAssociated with Ravi C. Dwivedi