Isabel Madrid

Experience

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  University of Málaga

  Apr 2008 - Jul 2008

  Research assistant (Internship)

  - Immunohistochemistry for bright field microscopy and fluorescence - Histological microtome-frozen and paraffin sections- Work with laboratory animals (perfussion and isolation tissue)

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  Institut de Bioenginyeria de Catalunya (IBEC)

  Feb 2009 - Dec 2010

  Lab Manager

  -Maintenance laboratory area and cell cultures (preparation buffers, stocks, culture media ...) - Management and biological sampling of mice for subsequent genotyping using PCR. -Support management of colonies of mice in animal facilities (breeding, feeding, husbrandy)-Administration laboratory (ordering, database management, accounting)

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  Universidad de Córdoba

  Jun 2011 - Feb 2012

  Lab technician

  - Maintenance of laboratory (preparation of buffers and culture media)- Stock management and ordering.- Working from samples of the fungus Fusarium (growing and plate, collection of spores and mycelium, extraction of genomic DNA, quantification in agarose gel electrophoresis and Nanodrop)-Bacteria cultures (Selection, picking up and plating of recombinant colonies, transformation and DNA extraction)

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  Semiramis Genetics Ltd

  Jan 2013 - Apr 2013

  Office Manager

  -Marketing, selling and shipping Non-hazardous Laboratory Reagents-Stock management and products preparation-Ordering, accounting and database management

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  University of Cambridge

  Apr 2013 - Sept 2016

  Chief Laboratory Research Technician

  - Cell culture with haematopoyetic precursos (cells in suspension) and adherent cells ( 293Tfor transfection and B16 for collection of media with grow factors)-Assays: transfection of adherent cells (293T cell line) with pGL2-Basic and pCL-Eco Retrovirus; cellular differentiation with cytokines, killing curve for antibiotics; luciferase test; single cell sorting for cell cloning selection and induction tests with ligando; ISH (in situ hybridization);Crispr-Cas9 screening; stable and transient transfection of haematopoyetic precursors (HOXB8-FL), mieloids cells (416B) and mastocytes (FMP6- and MST cell lines) by electroporation; nucleai isolation of HOXB8FL and 416B cells for Chip-Cloning: Production of competent cells, ligation, bacteria transformation (electroporation and thermic shock), DNA extraction with columns (Macherey-Nagel Kit,Maxis and Minipreps), digestion with restriction enzymes, agarose band purification -Flow cytometry (using Cyan ADP, Beckman Coulter) and introduction to BD LSR Fortessas -Q-PCR and RT-PCR (M- MLV Kit)-Histology: paraffin inclusion of mice embryos, microtome sectioning, fixing, staining, microscopy (bright field).-Implementations and update of Risk assessment protocols, ordering through University of Cambridge marketplace Show less

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  Aglaris Ltd.

  Sept 2016 - Apr 2017

  Laboratory Manager

  -Communication with suppliers for creating accounts, asking and negotiating quotes, ordering, receiving and stocking laboratory reagents, consumables, equipment and engineering materials-Induction management of new staff and hosting visitors-Cell culture with adherent cells in flask and microcarriers

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  Sartorius Stedim Biotech

  Jun 2017 - Jan 2018

  Development Scientist

  -Working on the development Ambr 250 high throughput perfusion system and late stage development of AMbr 250 fermentation and modular units.-Planning mammalian cells, bacteria and yeast culture to test the different Ambr systems

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  Autolus Ltd.

  Jan 2018 - Jan 2022

  Provide line management for a team of operations associates and scientists. Supervise the activities on the assigned shift. Provide input to employee performance management process.Ensure that team members follow defined GMP standards and procedures and comply with quality requirements, including records completion and training updates.Investigate and find root cause of deviations and provide technical expertise.Coordinate the activities in production prioritizing production schedules.Assist in the training of new personnel. Show less -Helping to establish a manufacturing site for 4 clinical trials, up to 200 batches per year-Performed ATMP manufacturing for clinical trials in grade B and C, ensuring all manufacturing activities are undertaken in accordance with production requirements and GMP regulatory and quality standards.-Assisted in coordination of production scheduling activity and distribution of materials and final drug products.-Created and maintained compliant process documentation (URS, IA, DQ, IQOQ, SOPs).-Complete validation work in conjunction with the QA department for the commissioning of new equipment or implementation of manufacturing methods and practices.- Supervised, trained and line managed junior staff.-Supported GMP activity for the manufacture of ATMPs to facilitate technology transfer, process validation, equipment validation and qualification, stability studies and materials management. Show less

  • Product Steward, Manufacturing Science and Technology

    Oct 2020 - Jan 2022

  • Production Team Leader

    Jan 2020 - Oct 2020

  • Production Scientist, Technical Operations

    Jan 2018 - Jan 2020
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  Moderna

  Jan 2022 - now

  • Senior Manager, Manufacturing Operations

    Jan 2023 - now

  • Manager Manufacturing Operations

    Jan 2022 - Jan 2023