Experience

• 

  Brigham and women's hospital,

  May 2005 - Oct 2010

  Research technician

  Key Responsibilities & Achievements• Conducted experiments on the role of CREB-binding protein in memory formation, adhering to SOPs while performing cellular and molecular analyses. Utilized advanced methodologies, including brain dissection, primary neuronal cell culture, PCR, fluorescence immunohistochemistry, histology, and Western blotting. Proficient in confocal and fluorescence microscopy, including Zeiss LSM 510 and Olympus BX50.• Managed over 30 transgenic mouse lines, overseeing breeding and colony maintenance in a clean animal facility while strictly following Home Office regulations. Analyzed PCR results for complex genotypes and maintained detailed breeding records.• Developed and optimized protocols for genotyping, Western blotting, histology, and immunohistochemistry.• Provided technical support to postdocs and collaborated effectively with team members to define project requirements, set priorities, and meet deadlines.• Oversaw laboratory operations, including equipment maintenance, consumables procurement, and reagent preparation.• Conducted COSHH and risk assessments, ensuring proper labeling and disposal of hazardous materials, including chemicals, sharps, and biological tissues, in full compliance with safety regulations. Assisted in internal and external lab inspections with health and safety officers.• Regularly participated in team meetings to present experimental findings and discuss project strategies. Attended workshops and training sessions to stay current with industry advancements and best practices.• Proficient in data analysis and visualization tools, including Microsoft Office (Word, Excel, PowerPoint) and ImageJ.• Maintained detailed records of antibodies and chemicals for accurate documentation and efficient resource management. Show less

• 

  The university of edinburgh

  Oct 2011 - now

  Professor Alexander Medvinsky Lab, Centre for Regenerative Medicine, Institute for Regeneration and Repair, Edinburgh UniversityKey Responsibilities & Achievements• Research Project Contribution: Generated transplantable CXCL10-genetically modified human embryonic stem cells using CRISPR/Cas9 and PiggyBac systems, ensuring compliance with SOPs. Conducted molecular and morphological analyses, including PCR, qRT-PCR, fluorescence immunohistochemistry, and flow cytometry.• Genotyping & Molecular Techniques: Performed genotyping of multiple mouse lines to support various projects. Prepared and optimized constructs (transformation, purification, restriction enzyme digestion) for group members.• Training & Supervision: Trained students in molecular biology techniques and cell culture, ensuring protocol adherence. Provided P&M training to a junior technician, improving lab efficiency.• Laboratory Management: Maintained equipment, inventory (-20°C, -80°C, liquid nitrogen storage), and the cell culture facility. Conducted COSHH and risk assessments, ensuring safe disposal of hazardous materials and compliance with safety regulations.• Data & Record Keeping: Managed databases for cell lines, plasmids, antibodies, and chemicals. Attended weekly meetings to discuss projects and present results.• Software Proficiency: Experienced with Microsoft Office (Word, Excel, PowerPoint), GraphPad Prism, SnapGene, ImageJ, and FlowJo.• Collaboration & Workflow Optimization: Worked independently and within a team to prioritize tasks and meet deadlines effectively. Show less Professor Rose Zamoyska’s Group, Institute of Immunology and Infection, Edinburgh UniversityKey Responsibilities & Achievements• Immunology Research & Gene Editing:Studied eIF5a’s role in IFNγ production and cell cycle regulation in CD8+ T cells.Performed molecular and cellular analyses, including tissue dissection, cell culture, CRISPR/Cas9 gene editing, PCR, qRT-PCR, fluorescence immunohistochemistry, Western blotting, and flow cytometry.• Cell Culture & Facility Maintenance:Cultured multiple cell lines, including HEK293T, F5 hybridoma, EL4, B16F10, Jurkat, human PBMCs, Phoenix Ampho, M38 hybridoma, lymphocytes, and Plat-E.Managed stock, reagents, and weekly maintenance for a clean and organized cell culture facility.• Molecular Biology & Genotyping:Developed and optimized PCR-based genotyping protocols for transgenic mouse lines.Provided technical support in molecular biology to postdocs, PhD students, and research assistants.• Laboratory Operations & Equipment Management:Streamlined lab operations, including ordering equipment and consumables.Maintained and calibrated the Miltenyi Biotec MACSQuant Analyzer 10, coordinating servicing and troubleshooting.• Health & Safety Compliance:Conducted COSHH and risk assessments for experiments.Ensured proper labeling and disposal of hazardous materials in compliance with regulations.Assisted in internal and external lab inspections with health and safety officers.• Collaboration & Knowledge Sharing:Presented experimental results in weekly meetings and contributed to project discussions.Attended training sessions and workshops to stay current with advancements in molecular and cell biology techniques.• Data Analysis & Record-Keeping: Proficient in Microsoft Office (Word, Excel, PowerPoint), GraphPad Prism, SnapGene, NCBI Primer-BLAST, CHOPCHOP, and FlowJo.Maintained detailed records of cell lines, plasmids, antibodies, and chemicals for efficient resource tracking. Show less

  • Senior Research Technician

    Apr 2022 - now

  • Research Technician

    Oct 2011 - Apr 2022