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Sheniqua Brown, PhD

Sheniqua Brown, PhD

Summer Intern

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location of Sheniqua Brown, PhDLanham, Maryland, United States

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  • Timeline

  • About me

    Principle Systems Security Engineer

  • Education

    • University of Maryland Baltimore County

      2012 - 2015
      Master’s Degree Chemical and Biochemical Engineering 3.264/4.0

      Activities and Societies: Meyerhoff Graduate Fellowship, Bridges to Doctorate Fellowship My master's thesis focused on developing a non-invasive fluorescent protein sensor for measuring glucose levels in neonates. The initial steps include the production and analysis of glucose binding protein (GBP) which involves cell culture and fermentation of modified E. coli, extracting GBP from cells, fluorescently labeling protein and purifying GBP using size exclusion and ion exchange chromatography. Other analytical techniques involved in my research include SDS-PAGE, Western Blot, and… Show more My master's thesis focused on developing a non-invasive fluorescent protein sensor for measuring glucose levels in neonates. The initial steps include the production and analysis of glucose binding protein (GBP) which involves cell culture and fermentation of modified E. coli, extracting GBP from cells, fluorescently labeling protein and purifying GBP using size exclusion and ion exchange chromatography. Other analytical techniques involved in my research include SDS-PAGE, Western Blot, and the execution of protein assays using fluorescence spectrometer. Additional studies included determining the bioactivity of GBP in various pH and ionic strength buffer conditions using quenching and lifetime experiments. Ultimately, the bioactivity experiments will help to determine the storage conditions, as well as the operating and testing conditions for the protein sensor. Show less

    • University of Maryland Baltimore County

      2015 - 2019
      Doctor of Philosophy (Ph.D.) Chemical and Biochemical Engineering

      Activities and Societies: LSAMP Bridges to the Doctorate, Meyerhoff Graduate Fellowship My research focuses on the development of non-invasive glucose sensor for neonates using fluorescently labeled protein.

    • Howard University

      2008 - 2012
      Bachelor of Science (B.S.) Chemical Engineering 3.96

      Activities and Societies: Tau Beta Pi, Society of Collegiate Scholars, American Institute of Chemical Engineers

  • Experience

    • 3P Incorporated

      Jun 2009 - Jul 2009
      Summer Intern

      Interned for a consulting firm specializing in project, process, and performance services in information technology, construction management and training for local and international clients. Performed market research and proposal writing for information technology division. Assisted with implementing various marketing and promotional strategies for customize service packages to state, local, federal and international government agencies

    • Howard University

      Jun 2010 - May 2012

      Tutor undergraduate students in calculus, differential equations and college algebra. Researched and studied the synthesis and behavior of silver nanoparticles. Developed skills needed to use Dynamic Light Scattering Device and Ultra-Violet Visible Spectrophotometer. Used these instruments to study the bioactivity of silver nanoparticles under various conditions.

      • Mathematics Tutor

        Jan 2010 - May 2012

      • Summer Researcher

        Jun 2010 - Aug 2010

    • Merck

      Jun 2011 - Aug 2011
      Summer Manufacturing Intern

      Worked in the Merck Manufacturing Division (MMD) in sterile liquid operations. Developed a procedure and tested alternative equipment used in the filtering process of vaccines in their early production stages. Conducted validation testing for machinery utilized in various sterile operation processes. Utilized and enhanced skills in process optimization.

    • University of Maryland Baltimore County

      Jun 2012 - May 2019

      I performed research in UMBC’s Center for Advanced Sensor Technology under Dr. Govind Rao and Dr. Leah Tolosa. My research involves developing a non-invasive glucose sensor that uses fluorescent labeled glucose binding protein. The goal is to develop a commercial sensor that can monitor glucose levels in individuals suffering from diabetes, including neonates. The initial steps include the production and analysis of GBP which involves cell culture and fermentation of modified E. coli, extracting GBP from cells, fluorescent labeling of protein and purifying GBP using size exclusion and ion exchange columns. Other techniques involved in my research include SDS-Page, Bradford Protein Assay, Western Blot and the use of a fluorescence spectrometer. Additional research being performed includes studying the bioactivity of GBP in various pH and ionic strength buffer conditions using quenching and lifetime experiments. Ultimately, the bioactivity experiments will help to determine the storage conditions, as well as the operating and testing conditions for the protein sensor. I'm also doing work with plasmids which include plasmid purification, transformation with competent cells, inoculation and various expression studies. Show less

      • Graduate Research Assistant

        Jun 2012 - May 2019

      • Summer Research Assistant

        Jun 2012 - Jul 2012

    • Northrop Grumman

      Aug 2019 - now
      Principle Systems Security Engineer

  • Licenses & Certifications

    • Graduate Certificate in Biochemical Regulatory Engineering

  • Honors & Awards

    • Awarded to Sheniqua Brown, PhD
      UMBC LSAMP Bridge to the Doctorate Fellowship - Aug 2012
    • Awarded to Sheniqua Brown, PhD
      UMBC Meyerhoff Graduate Fellow - Aug 2012
    • Awarded to Sheniqua Brown, PhD
      Howard University College of Engineering, Architecture and Computer Science Academic Achievement Award- 2nd Highest GPA Howard University May 2012
    • Awarded to Sheniqua Brown, PhD
      Howard University Department of Chemical Engineering Outstanding Achievement Award - May 2012
    • Awarded to Sheniqua Brown, PhD
      Tau Beta Pi Fellowship - May 2012